← Vitamins & Injectables

Vitamin D3

Cholecalciferol is converted in the liver and kidney to calcitriol, the active hormone regulating calcium absorption, bone metabolism, and immune signaling.

Dr. Jonathan Snipes, MDMedically reviewed by Dr. Jonathan Snipes, MD. Last reviewed May 6, 2026.
Learn the Science ↓
Vitamin D3 injectable vial compounded by a licensed U.S. 503A compounding pharmacy for Rx Peps Direct

Verify us before you order

High DoseCorrects DeficiencyBone & ImmuneSelf-Inject

What It Is

An injectable form of vitamin D3 (cholecalciferol) at 50,000 IU/mL. Self-administered intramuscular, typically dosed weekly to monthly as directed by your provider.

How It Works

Injected D3 is hydroxylated to 25-hydroxyvitamin D and then to active calcitriol, supporting calcium and phosphate homeostasis, bone mineralization, and immune function. Injection helps patients with malabsorption or persistent deficiency on oral D3.

Who It's For

Patients with documented vitamin D deficiency or malabsorption who have not responded to oral supplementation.

Who Should Avoid It

Hypercalcemia, hypervitaminosis D, or sarcoidosis-related calcium dysregulation. Do not stack multiple high-dose D sources. Flag for medical review.

Ready to add Vitamin D3?

A licensed provider reviews every order.

Protocol & Pricing

Pharmacy Price

$55/30ml

You pay pharmacy price. No markup.

Starting Dose

50,000 IU (1mL) once monthly

Form

Injectable vial (30mL, 50,000 IU/mL cholecalciferol)

Dosing Protocol

50,000 IU (1mL at 50,000 IU/mL) intramuscular once monthly, or per provider. Single 30mL vial ($55). Do not exceed provider-directed dosing.

Beyond Use Date

60 Days from compounding (USP <797>)

A licensed U.S. 503A compounding pharmacy assigns this date and prints it on your vial label. Always follow the label.

Ready to add Vitamin D3?

A licensed provider reviews every order.

Stacking Guide

Stacks Well With

Methylcobalamin (B12)Vitamin B-Complex

Ready to add Vitamin D3?

A licensed provider reviews every order.

Related Peptides

Methylcobalamin (B12)

Injectable methylcobalamin, the active, ready-to-use form of vitamin B12 for energy and methylation support.

$30/10ml

Vitamin B-Complex

Injectable B-complex (B1, B2, B3, B6) for energy metabolism and nervous system support.

$40/30ml

Independently tested

Vitamin D3 certificate of analysis

All 2 Vitamin D3 samples tested for sterility and endotoxin passed both. Latest sample received by the lab Sep 23, 2026.

Every Vitamin D3 batch dispensed by a licensed U.S. 503A compounding pharmacy is independently tested by Eagle Analytical Services (FDA-registered, A2LA ISO 17025 accredited). Sterility testing checks that nothing grows in the sample; the USP <85> endotoxin test checks for bacterial toxins that can cause fever. Below is the lab's own report for each of the 2 most recent lots.

Lot LG342010871, received Sep 23, 2026

Sterility
PassCelsis Sterility Test. Started Sep 24, 2026, completed Oct 2, 2026. Method suitability ETX-250809-0008
Bacterial endotoxin
PassUSP <85>. Measured <2 EU/mL, limit 700 EU/mL. Started Sep 25, 2026, completed Sep 26, 2026
Sample, as printed
VITAMIN D3 50,000 IU/ ML (30 ML) Injection
Lot #
LG342010871
Storage
Room Temperature
Received by lab
Sep 23, 2026
Laboratory
Eagle Analytical Services Inc., Houston, TX
Submission #
ETX-260923-0337
Signed
Alison Eng, Quality Control Manager
Scan of Eagle Analytical report ETX-260923-0337 for lot LG342010871. Opens the original PDF.
Eagle Analytical report ETX-260923-0337. Open the original PDF (opens in a new tab)

Eagle Analytical Services is an independent FDA-registered laboratory, A2LA accredited to ISO 17025, based in Houston, Texas. They have no commercial relationship with Rx Peps Direct. Rx Peps Direct prescribes; the pharmacy compounds and dispenses; Eagle tests every batch. Three separate companies. Results apply only to the tested sample as received. Browse our full quality program on the quality page.

Ready to add Vitamin D3?

A licensed provider reviews every order.

How to get Vitamin D3 prescribed online

Vitamin D3 is available by prescription after an online medical review and dispensed for human use by a licensed 503A pharmacy. That is what separates it from research-use-only vials sold without a prescription. There is no membership, just a medical review fee per order, and automatic refills are optional.

  1. 1

    Complete a $39 medical review. Answer a short intake about your health history and your goals for Vitamin D3. The $39 fee is the only charge Rx Peps Direct collects, paid once, not a recurring subscription.

  2. 2

    A licensed provider reviews it. A US-licensed clinician reviews your intake and, if Vitamin D3 is appropriate for you, writes a patient-specific prescription. The starting dose is typically 50,000 IU (1mL) once monthly.

  3. 3

    The pharmacy compounds and ships it. A licensed 503A compounding pharmacy in Texas compounds Vitamin D3 and ships it pre-reconstituted by FedEx overnight in a reusable cooled case. Your fill is Injectable vial (30mL, 50,000 IU/mL cholecalciferol), and you pay the pharmacy $55/30ml at wholesale, with no clinic markup, plus a $15 flat FedEx overnight shipping fee charged by the pharmacy.

Rx Peps Direct prescribes Vitamin D3 in the 28 states plus Washington, DC where its medical team is licensed. Start your $39 medical review or browse the full Vitamins & Injectables range. Comparing services first? See how Rx Peps Direct stacks up against seven other providers in our best telehealth for compounded peptides comparison.

Ready to add Vitamin D3?

A licensed provider reviews every order.

Common questions about Vitamin D3

What is Vitamin D3?
An injectable form of vitamin D3 (cholecalciferol) at 50,000 IU/mL. Self-administered intramuscular, typically dosed weekly to monthly as directed by your provider.
How does Vitamin D3 work?
Injected D3 is hydroxylated to 25-hydroxyvitamin D and then to active calcitriol, supporting calcium and phosphate homeostasis, bone mineralization, and immune function. Injection helps patients with malabsorption or persistent deficiency on oral D3.
Who is Vitamin D3 for?
Patients with documented vitamin D deficiency or malabsorption who have not responded to oral supplementation.
How much does Vitamin D3 cost?
Vitamin D3 costs $55/30ml from a licensed U.S. 503A compounding pharmacy at wholesale, with a flat $39 medical review from Rx Peps Direct. The form is Injectable vial (30mL, 50,000 IU/mL cholecalciferol).
What is the Beyond Use Date for Vitamin D3?
A licensed U.S. 503A compounding pharmacy assigns the Beyond Use Date for Vitamin D3 and prints it on the vial label. The listed figure is 60 Days from compounding (USP <797>). Always follow the date on your own label.

Ready to add Vitamin D3?

A licensed provider reviews every order.